A lightcycler probe system consists of a pair of singlestranded fluorescentlabeled oligonucleotides. Roche applied science lightcycler 480 realtime pcr system. You can now create experimental protocols more quickly by using templates and macros. Enter username and password when the login dialog box appears and click the button to proceed with the login. The factor ii prothrombin g20210a test is an in vitro diagnostic test for the detection and genotyping of the factor ii prothrombin g20210a mutation as an aide to dignosis in the evaluation of patients with suspected thrombophilia. The modern instrument design, outstanding technical and software features, as well.
May 17, 20 the lightcycler nano system features a plugandplay device with stylish design, making it the smallest and most silent realtime pcr system on the market. Information specific for single kits can be found in the individual manuals for the respective kits. The lightcycler was licensed to roche in 1997, and was unveiled to the world in 1998. Realtime ready custom panels create your specific gene expression panel with realtime ready assays of your choice on lightcycler 480 multiwell plates 96 or 384, using the realtime ready configurator. I have a problem with the analysis in lightcycler 480 software. Navigate the list of programs until you find lightcycler 480 sw 1. Nano software is an application offered by roche diagnostics. The test is intended to be used on the lightcycler 2. When used with the lightcycler 480 system, this kit is ideally suited for hot start pcr.
Additional features of the lightcycler 480 pcr system. Realtime ready assays are available as single assays for the selected target or preplated on lightcycler 480 multiwell plates 96 or 384. These machine are great fluorometers, they could be used for other purposes than. Warranty information on warranty conditions are specified in the sales contract. Nano software from your pc using advanced uninstaller pro lightcycler. Without prior incubation or culture, whole blood 1. Set all other protocol parameters not listed in the table below to 0. Opening new horizons in the field of data analysis. Evaluation of the lightcycler methicillinresistant. Pcr primer and probe design software used with all lightcycler carouselbased instruments and lightcycler 480 instruments. The complete security suite for all your datacommunication needs invisible secrets encryption software 4 not only encrypts your data and files for safe keeping or for secure transfer across the net, it also hides them in places that on the surface appear totally innocent, such as picture or sound files, or web pages. The cycler has 4 detection channels, which detect hydrolysis 5nuclease taqman probes either as single color or multiplex samples.
A novel fret pair for detection of parallel dna triplexes. Customizable modular system concept building on the principles of the wellestablished, performanceproven lightcycler software 4. It is defined as 30 cycles completed in 10 to 30 minutes. Class 2 device recall factor ii prothrombin g20210a kit. Applied biosystems 7500 realtime pcr system thermo. Create your specific gene expression panel with realtime ready assays of your choice on lightcycler 480 multiwell plates 96 or 384, using the realtime ready configurator a multitude of different layouts is available for configuration. Lightcycler software 4, free lightcycler software 4 software downloads. The lightcycler septifast test is ceivd marked and identifies the 25 most common pathogens known to cause sepsis, including 10 gramnegative bacteria, 9 grampositive bacteria, and 6 fungal agents. Oligo probe 1 is labeled at the 3 end with a donor reporter and oligo probe 2 is labeled at its 5 end with one of a few available acceptor reporters.
Roche lightcycler 480 ii real time pcr system for 96 well. Free lightcycler software download download lightcycler. Exporting lightcycler software 4 experiments and templates. You clicked on an external link, which has been disabled in order to keep your shopping session open. Roche molecular biochemicals lightcycler operators manual. Lightcycler 480 software provides two methods for performing absolute quantification. The colour compensations were performed according to the lightcycler 2. The only difference between this updated version and lightcycler software 4. Depending on the red fluophore dye used for labeling the hybprobe probe, choose channel 610, 640, 670 or 705. For additional information on calibrating the lightcycler 2. Roche lightcycler 480ii384 pcr w thermocycler 96s 2. New file format for roche devices lightcycler 480 has been added.
Lightcycler 480 realtime pcr system from roche applied. Over 20,000 lightcyclers have been sold, providing a rapid option for realtime pcr in 1530 min. Turn on the computer linked to the instrument and log on to window xp. Has anybody used the light cycler 480 system by roche. The first fda approved kits for genetic variants factor v leiden and prothrombin appeared on the lightcycler in 2003, and a 6color version the lc 2. A novel fret pair for detection of parallel dna triplexes by. The lightcycler 480 system unleash the potential of realtime pcr. Draggable legend and ability to not display it or on several. The platebased lightcycler 480 system is a highly adaptable and versatile realtime pcr system for the analysis of gene expression and genetic variation. Continue from step 5 of the 5 nuclease procedure listed above. It integrates all software modules required to perform and analyze any type of experiment using the lightcycler 2. Close the instrument and start the lightcycler software application 4.
Methicillinresistant staphylococcus aureus mrsa has been established as a health careassociated pathogen in many countries worldwide since the 1980s 1, 2. However, it is essential that the fluorescent dyes used are compatible with the lightcycler 2. Lightcycler software 4 software free download lightcycler. Nasal colonization is known to be a risk factor for the development of mrsa. Intended use these instructions are intended for use of eurofins genescan lr, nr, umm kits on roche lightcycler 480 i and ii. The lightcycler 480 system unleash the potential of real. The cycle number at which the temperature step up or step. Roche life science realtime ready overview lightcycler. Roche lightcycler 96 biotecon diagnostics biotecon. Due to differences in instrument platforms, new programming procedures for the lightcycler 480 instrument ii software 1. Lightcycler program validation for lightcycler program validation the antiparallel duplex probes were measured at ph 6, 7 and 8 and the parallel triplex probes were measured at ph 5, 5.
Maybe the downside is the lightcycler software that is somehow outdated. Contact your roche representative for further information. Free lightcycler software download download software at updatestar. The information on this page is only about version 1. I prepared the twostep cycling experiment with the following conditions. All the programs existing on your computer will be made available to you 6. It offers fast and accurate analysis that impresses with its intuitive and userfriendly software. For lightcycler program validation the antiparallel duplex probes were measured at ph 6, 7 and 8 and the parallel triplex probes were measured at ph 5, 5.
Roche lightcycler 480 ii real time pcr system for 96 well plates 8856. Reports of mrsa carriage or acquisition in the community have become a major focus of medical and public concern during the past decade 3, 4. Lightcycler sample carousel lightcycler capillaries lightcycler centrifuge adapters lightcycler software package cat. Conforms to the european directive for in vitro diagnostic medical devices 9879ec in combination with the lightcycler 2.
Lightcycler 480 instrument operators manual software version 1. Software, transmission and storage, patient data product code nsx product lightcycler 2. For technical support on the quidel molecular direct c. Disclaimer of license no rights to perform the polymerase chain reaction pcr process, including homogeneous or realtime pcr methods, covered by patents owned by roche molecular sys. The following instructions are based on lightcycler 480 software version 1. After youve downloaded crossover check out our youtube tutorial video to the left, or visit the crossover chrome os walkthrough for specific steps.
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